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Kinetic modeling and sensitivity analysis of acetone–butanol–ethanol production

Paper ID Volume ID Publish Year Pages File Format Full-Text
25208 43560 2007 12 PDF Available
Title
Kinetic modeling and sensitivity analysis of acetone–butanol–ethanol production
Abstract

A kinetic simulation model of metabolic pathways that describes the dynamic behaviors of metabolites in acetone–butanol–ethanol (ABE) production by Clostridium saccharoperbutylacetonicum N1-4 was proposed using a novel simulator WinBEST-KIT. This model was validated by comparing with experimental time-course data of metabolites in batch cultures over a wide range of initial glucose concentrations (36.1–295 mM). By introducing substrate inhibition, product inhibition of butanol, activation of butyrate and considering the cessation of metabolic reactions in the case of insufficiency of energy after glucose exhaustion, the revised model showed 0.901 of squared correlation coefficient (r2) between experimental time-course of metabolites and calculated ones. Thus, the final revised model is assumed to be one of the best candidates for kinetic simulation describing dynamic behavior of metabolites in ABE production. Sensitivity analysis revealed that 5% increase in reaction of reverse pathway of butyrate production (R17) and 5% decrease in reaction of CoA transferase for butyrate (R15) highly contribute to high production of butanol. These system analyses should be effective in the elucidation which pathway is metabolic bottleneck for high production of butanol.

Keywords
Acetone–butanol–ethanol production; Clostridium saccharoperbutylacetonicum N1-4; WinBEST-KIT; Kinetic model; On–off mechanism; Sensitivity analysis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 131, Issue 1, 1 August 2007, Pages 45–56
Authors
, , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us