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Setting up and modelling of overflowing fed-batch cultures of Bacillus subtilis for the production and continuous removal of lipopeptides

Paper ID Volume ID Publish Year Pages File Format Full-Text
25210 43560 2007 9 PDF Available
Title
Setting up and modelling of overflowing fed-batch cultures of Bacillus subtilis for the production and continuous removal of lipopeptides
Abstract

This work is related to the set-up of overflowing exponential fed-batch cultures (O-EFBC) derived from carbon limited EFBC dedicated to the production of mycosubtilin, an antifungal lipopeptide belonging to the iturin family. O-EFBC permits the continuous removal of the product from the bioreactor achieving a complete extraction of mycosubtilin. This paper also provides a dynamical Monod-based growth model of this process that is accurate enough to simulate the evolution of the specific growth rate and to correlate it to the mycosubtilin specific productivity. Two particular and dependant phenomena related to the foam overflow are taken into account by the model: the outgoing flow rate of a broth volume and the loss of biomass. Interestingly, the biomass concentration in the foam was found to be lower than the biomass concentration in the bioreactor relating this process to a recycling one. Parameters of this model are the growth yield on substrate and the maximal specific growth rate estimated from experiments led at feed rates of 0.062, 0.071 and 0.086 h−1. The model was extrapolated to five additional experiments carried out at feed rates of 0.008, 0.022, 0.040, 0.042 and 0.062 h−1 enabling the correlation of the mean specific growth rates with productivity results. Finally, a feed rate of 0.086 h−1 corresponding to a mean specific growth rate of 0.070 h−1 allowed a specific productivity of 1.27 mg of mycosubtilin g−1 of dried biomass h−1.

Keywords
Bacillus subtilis; Biosurfactant lipopeptides; Mycosubtilin; Foaming; Exponentially fed-batch culture; Parameters identification
First Page Preview
Setting up and modelling of overflowing fed-batch cultures of Bacillus subtilis for the production and continuous removal of lipopeptides
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 131, Issue 1, 1 August 2007, Pages 67–75
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering