fulltext.study @t Gmail

A highly stable cambialistic-superoxide dismutase from Antrodia camphorata: Expression in yeast and enzyme properties

Paper ID Volume ID Publish Year Pages File Format Full-Text
25212 43560 2007 8 PDF Available
Title
A highly stable cambialistic-superoxide dismutase from Antrodia camphorata: Expression in yeast and enzyme properties
Abstract

A cDNA encoding a putative superoxide dismutase (SOD) was identified in expressed sequence tags of Antrodia camphorata, a medicinal mushroom found only in Taiwan. The deduced protein was aligned with Mn-SODs and Fe-SODs from other organisms, this SOD showed greater homology to Mn-SOD. Functional A. camphorata SOD protein was overexpressed in yeast and purified. The purified enzyme showed two active forms on a 12.5% native PAGE, a dimer and a monomer. The dimeric protein's half-life of deactivation at 80 °C was 7 min, and its thermal inactivation rate constant Kd was 9.87 × 10−2 min−1. The enzyme was stable in a broad pH range from 5–11; in the presence of 0.4 M imidazole and 2% SDS. The atomic absorption spectrometric assay showed that 1.0 atom of manganese/iron (9:1) was present in each SOD subunit. The high stability of the enzyme make it better suited than other cambialistic-SODs for use in cosmetics. The SOD also documents its future utility in developing anti-inflammatory agent and in the treatment of chronic diseases.

Keywords
Mushroom; Antrodia camphorata; Expression; Cambialistic-superoxide dismutase (Mn/Fe-SOD); Yeast
First Page Preview
A highly stable cambialistic-superoxide dismutase from Antrodia camphorata: Expression in yeast and enzyme properties
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 131, Issue 1, 1 August 2007, Pages 84–91
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us