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Bacterial protein patterning by micro-contact printing of PLL-g-PEG

Paper ID Volume ID Publish Year Pages File Format Full-Text
25219 43561 2007 6 PDF Available
Title
Bacterial protein patterning by micro-contact printing of PLL-g-PEG
Abstract

Biomimetic micro-patterned surfaces of three S-layer (fusion) proteins, wild type (SbpA), enhanced green fluorescence protein (SbpA-EGFP) and streptavidin (SbpA-STV), were built by microcontact printing of poly-l-lysine grafted polyethylene glycol (PLL-g-PEG). The functionality of the adsorbed proteins was studied with atomic force microscopy and fluorescence microscopy. Atomic force microscopy (AFM) measurements showed that wild-type SbpA recrystallized on PLL-g-PEG free areas, while fluorescent properties of SbpA-EGFP and the interaction of SbpA-streptavidin heterotetramers with biotin were not affected due to the adsorption on the micro patterned substrates.

Keywords
S-layer fusion protein; Micro patterning; Enhanced green fluorescent protein; Streptavidin; Atomic force microscopy; Biomimetic surfaces
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Bacterial protein patterning by micro-contact printing of PLL-g-PEG
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 130, Issue 3, 30 June 2007, Pages 247–252
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us