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Production of a Rhizopus oryzae lipase from Pichia pastoris using alternative operational strategies

Paper ID Volume ID Publish Year Pages File Format Full-Text
25224 43561 2007 9 PDF Available
Title
Production of a Rhizopus oryzae lipase from Pichia pastoris using alternative operational strategies
Abstract

Different cultivation strategies have been compared for the production of Rhizopus oryzae lipase (ROL) from Pichia pastoris. Several drawbacks have been found using a methanol non-limited fed-batch. On the one hand, oxygen limitation appeared at early cell dry weights and, on the other hand, high cell death was observed. A temperature limited fed-batch has been proposed to solve both problems. However, in our case study a methanol non-limited fed-batch results in better productivities. Finally, a lower salt medium were used to overcome cell death problems and a temperature limited fed-batch was applied thereafter to solve oxygen transfer limitations. This combined strategy has resulted in lower productivities when compared to a methanol non-limited fed-batch. However the culture could be longer prolonged and a 1.3-fold purer final product was obtained mainly due to cell death reduction.

Keywords
Pichia pastoris; Operational fed-batch strategies; Rhizopus oryzae lipase; Cell death
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Production of a Rhizopus oryzae lipase from Pichia pastoris using alternative operational strategies
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 130, Issue 3, 30 June 2007, Pages 291–299
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us