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A novel three-dimensional aerogel biochip for molecular recognition of nucleotide acids

Paper ID Volume ID Publish Year Pages File Format Full-Text
2523 114 2010 9 PDF Available
Title
A novel three-dimensional aerogel biochip for molecular recognition of nucleotide acids
Abstract

Mesoporous aerogel was produced under regular atmospheric conditions using the sol–gel polymerization of tetraethyl orthosilicate with an ionic liquid as both solvent and active agent. This was then used to build a three-dimensional structure to recognize nucleotide acids. Fourier transformation infrared spectroscopy, scanning electron microscopy, 29Si solid-state nuclear magnetic resonance, and Brunauer–Emmett–Teller instruments were used to characterize this 3D aerogel, demonstrating that it had high porosity and large internal networking surface area that could capture nucleotide acids. The functionality of molecular recognition on nucleotide acids was demonstrated by immobilizing an oligonucleotide to probe its DNA target and confirming the tagged fluorescent signals by confocal laser scanning microscopy. The results indicated that the as-prepared 3D bioaerogel was capable of providing a very large surface area to capture and recognize human gene ATP5O.

Keywords
Aerogel; Molecular recognition; DNA detection; 3D biochips
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A novel three-dimensional aerogel biochip for molecular recognition of nucleotide acids
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 6, Issue 4, April 2010, Pages 1462–1470
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us