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Regulation of transduction efficiency by pegylation of baculovirus vector in vitro and in vivo

Paper ID Volume ID Publish Year Pages File Format Full-Text
25241 43562 2006 6 PDF Available
Title
Regulation of transduction efficiency by pegylation of baculovirus vector in vitro and in vivo
Abstract

In this study, poly(ethylene glycol) (PEG) was coupled to baculovirus to regulate transduction efficiency of baculovirus in vitro and in vivo. The degree of pegylation in virions was measured by the loss of free amines via a fluorescamine-based assay. The efficiency of green fluorescent protein (GFP) expression was used to monitor transduction efficiency. As the results, the transduction efficiency in pegylated baculovirus was decreased with an increase of pegylation in baculovirus in vitro and in vivo. Interestingly, the transduction efficiency of the pegylated baculovirus for the lung and brain was increased compared with baculovirus itself possibly owing to increased stability of baculovirus by pegylation.

Keywords
Pegylation; Baculovirus; Green fluorescent protein (GFP); Transduction efficiency
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Regulation of transduction efficiency by pegylation of baculovirus vector in vitro and in vivo
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 125, Issue 1, 20 August 2006, Pages 104–109
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us