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Protein activity in bacterial inclusion bodies correlates with predicted aggregation rates

Paper ID Volume ID Publish Year Pages File Format Full-Text
25242 43562 2006 4 PDF Available
Title
Protein activity in bacterial inclusion bodies correlates with predicted aggregation rates
Abstract

Recent data show that protein aggregation as bacterial inclusion bodies does not necessarily imply loss of biological activity. Here, we investigate the effect of a large set of single-point mutants of an aggregation-prone protein on its specific activity once deposited in inclusion bodies. The activity of such aggregates significantly correlates with the predicted aggregation rates for each mutant, suggesting that rationally tuning the kinetic competition between folding and aggregation might result in highly active, inclusion bodies. The exploration of this technology during recombinant protein production would have a significant biotechnological value.

Keywords
Inclusion bodies; Recombinant protein expression; Protein aggregation; Protein folding; Escherichia coli
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Protein activity in bacterial inclusion bodies correlates with predicted aggregation rates
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 125, Issue 1, 20 August 2006, Pages 110–113
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us