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A novel flow based hollow-fiber blood–brain barrier in vitro model with immortalised cell line PBMEC/C1–2

Paper ID Volume ID Publish Year Pages File Format Full-Text
25244 43562 2006 15 PDF Available
Title
A novel flow based hollow-fiber blood–brain barrier in vitro model with immortalised cell line PBMEC/C1–2
Abstract

A flow based hollow-fiber in vitro model of the blood–brain barrier (BBB) was established. The immortalised porcine brain microvascular endothelial cell line PBMEC/C1–2 was cultured in a pulsatile hollow-fiber cartridge system (Cellmax Quad). The usability of PBMEC/C1–2 in the flow based hollow-fiber model was increased from three days in the originally used Transwell model up to four months due to the application of shear stress and co-culturing with glioma cell line C6. It was shown that the tightness of PBMEC/C1–2 layers was enhanced significantly in astrocyte conditioned medium (ACM) and in co-culture. The morphology of PBMEC/C1–2 and C6 was visualised by environmental scanning electron microscopy (ESEM). Permeation studies were accomplished with a set of benzodiazepines. The raw data were processed with three different calculation models and the results were compared with permeability coefficients obtained with an established Transwell model. In summary a flow based hollow-fiber BBB in vitro model was developed, which can be used to perform experiments with physiological (e.g., regulation of BBB permeability), pharmacological (e.g., pharmacokinetics and dynamics) and pathophysiological (e.g., effects of diseases on BBB permeability and vice versa) objectives.

Keywords
Blood–brain barrier; Flow based hollow-fiber in vitro model; PBMEC/C1–2; Benzodiazepines
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A novel flow based hollow-fiber blood–brain barrier in vitro model with immortalised cell line PBMEC/C1–2
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 125, Issue 1, 20 August 2006, Pages 127–141
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us