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Synthesis of structured phospholipids by immobilized phospholipase A2 catalyzed acidolysis

Paper ID Volume ID Publish Year Pages File Format Full-Text
25274 43564 2007 10 PDF Available
Title
Synthesis of structured phospholipids by immobilized phospholipase A2 catalyzed acidolysis
Abstract

Acyl modification of the sn-2 position in phospholipids (PLs) was conducted by acidolysis reaction using immobilized phospholipase A2 (PLA2) as the catalyst. In the first stage we screened different carriers for their ability to immobilize PLA2. Several carriers were able to fix the enzyme and maintain catalytic activity; however, the final choice of carrier for the continued work was a non-ionic weakly polar macroreticular resin. Response surface methodology was applied to evaluate the influence of substrate ratio, reaction temperature, and water addition during acidolysis reaction between caprylic acid and soybean phosphatidylcholine (PC). Reaction temperature and water addition had significant effect on acidolysis reaction, however no effect was observed for substrate ratio (mol caprylic acid/mol PC) in range tested. In general an inverse relationship between incorporation of caprylic acid and PC recovery was observed. Highest incorporation obtained during acidolysis reactions was 36%. Such incorporation could be obtained under reaction temperature, 45 °C; substrate ratio, 9 mol/mol caprylic acid/PC; water addition of 2%; 30 wt.% immobilized enzyme; and reaction time, 48 h. The yield under these conditions was however only 29%. Lysophosphatidylcholine (LPC) was the major by-product formed during the reaction. Incorporation of acyl donor into LPC was very low (<4%), which indicates that acyl migration is only a minor problem for PLA2 catalyzed synthesis reaction. Conjugated linoleic acid and docosahexaenoic acid were also tested as acyl donors, and were able to be incorporated into PC with 30 and 20%, respectively.

Keywords
Immobilization; PLA2 catalyzed synthesis; Response surface methodology; Solvent-free system; Structured phospholipids
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Synthesis of structured phospholipids by immobilized phospholipase A2 catalyzed acidolysis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 128, Issue 3, 20 February 2007, Pages 545–554
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us