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Synthesis of PHBV block copolymers driven by an oscillatory genetic network

Paper ID Volume ID Publish Year Pages File Format Full-Text
25281 43564 2007 23 PDF Available
Title
Synthesis of PHBV block copolymers driven by an oscillatory genetic network
Abstract

Artificial genetic networks constitute a powerful tool to achieve various biotechnological objectives. In this work, we propose the modification of an oscillatory genetic network, known as the repressilator, to drive synthesis of poly(3hydroxybutyrate-co-3hydroxyvalerate) (PHBV) block copolymer chains in recombinant Escherichia coli cells. To study the feasibility of this idea, we developed a detailed mathematical model describing the dynamics of the genetic network, which drive the formation of monomer units that are subsequently incorporated into actively growing block copolymer chains. Extensive simulation studies have shown that appropriate choice of the molecular characteristics of the network and manipulation of extracelllular conditions lead to tight control of both the micro- and macro-structures of the resulting block copolymer chains. Thus, the model can guide network design aiming at producing block copolymer structures with desirable characteristics.

Keywords
Poly(3-hydroxybutyrate-co-3-hydroxyvalerate); PHBV; Block copolymers; Mathematical modeling; Repressilator; Genetic network; Population balance; Molecular weight distribution
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Synthesis of PHBV block copolymers driven by an oscillatory genetic network
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 128, Issue 3, 20 February 2007, Pages 615–637
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us