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The rolB gene-induced overproduction of resveratrol in Vitis amurensis transformed cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
25287 43564 2007 12 PDF Available
Title
The rolB gene-induced overproduction of resveratrol in Vitis amurensis transformed cells
Abstract

Resveratrol is a stilbene, which prevents carcinogenesis at stages of tumor initiation, promotion and progression. In the present investigation, we developed cell cultures of wild-growing grape (Vitis amurensis Rupr.). The cultures produced low levels of resveratrol, up to 0.026% dry wt., i.e., comparable to levels reported for other plant cell cultures previously established. Different methods commonly used to increase secondary metabolite production (cell selection, elicitor treatments and addition of a biosynthetic precursor) only slightly enhanced cell productivity. Transformation of V. amurensis V2 callus culture by the rolB gene of Agrobacterium rhizogenes resulted in more than a 100-fold increase in resveratrol production in transformed calli. The rolB-transformed calli are capable of producing up to 3.15% dry wt. of resveratrol. We show that the capability to resveratrol biosynthesis is tightly correlated with the abundance of rolB mRNA transcripts. Tyrosine phosphatase inhibitors abolished the rolB-gene-mediated stimulatory effect, thus documenting for the first time the involvement of tyrosine phosphorylation in plant secondary metabolism.

Keywords
MeJA, methyl jasmonate; PAO, phenylarsine oxide; Phe, phenylalanine; PFP, p-fluoro-dl-phenylalanine; SA, salicylic acid; SNP, sodium nitroprusside; BAP, 6-benzylaminopurine; NAA, α-naphthaleneacetic acid; IAA, indoleacetic acid; 2,4-D, 2,4-dichlorophenoxy
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 128, Issue 3, 20 February 2007, Pages 681–692
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us