fulltext.study @t Gmail

Surprising production of a new urdamycin derivative by S. fradiae ΔurdQ/R

Paper ID Volume ID Publish Year Pages File Format Full-Text
25292 43565 2007 7 PDF Available
Title
Surprising production of a new urdamycin derivative by S. fradiae ΔurdQ/R
Abstract

A strain (S. fradiae ΔurdQ/R) with mutations in urdQ and urdR encoding a dTDP-hexose-3,4-dehydratase and a dTDP-hexose-4-ketoreductase, respectively, produces a new urdamycin analogue (urdamycin X) with changes in the polyketide structure. The structure of urdamycin X has been elucidated by NMR spectroscopy. Urdamycin X was not detectable, even in small amounts, in either S. fradiae ΔurdQ, in S. fradiae ΔurdR or in S. fradiae A0, a mutant lacking all glycosyltransferase genes. Complementation of S. fradiae ΔurdQ/R restored urdamycin A production indicating that the mutations did not cause any polar effect.

Keywords
PCR, polymerase chain reaction; dTDP, deoxythymidine phosphate; NMR, nuclear magnetic resonance; NAD(H), nicotinamide adenine dinucleotide; NADP(H), nicotinamide adenine dinucleotide phosphate; PMP, pyridoxamine 5′-phosphateStreptomyces fradiae; Secondary
First Page Preview
Surprising production of a new urdamycin derivative by S. fradiae ΔurdQ/R
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 130, Issue 1, 31 May 2007, Pages 32–38
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us