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Construction of thermotolerant yeast expressing thermostable cellulase genes

Paper ID Volume ID Publish Year Pages File Format Full-Text
25301 43566 2007 10 PDF Available
Title
Construction of thermotolerant yeast expressing thermostable cellulase genes
Abstract

Kluyveromyces marxianus NBRC1777 was identified as a thermotolerant yeast and was developed as a host for the expression of thermostable cellulase genes. The previously isolated genes for thermostable endo-β-1,4-glucanase, cellobiohydrolase, and β-glucosidase were introduced into the chromosome of K. marxianus and successfully expressed under the control of high-expression promoters. The recombinant K. marxianus expressing cellulase genes became able to grow in synthetic medium containing cellobiose or carboxymethyl-cellulose as the single carbon source. Moreover, the recombinant strain produced 43.4 g/L ethanol from 10% cellobiose. These results suggest that K. marxianus may afford a useful host system, which may be applicable to the simultaneous saccharification and fermentation and the foundation of cellulose consolidated bioprocessing.

Keywords
Kluyveromyces marxianus; Thermotolerant; Cellulose assimilation; Cellobiose-fermenting yeast
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Construction of thermotolerant yeast expressing thermostable cellulase genes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 130, Issue 2, 15 June 2007, Pages 114–123
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us