fulltext.study @t Gmail

Targeted deletion of genes encoding extracellular enzymes in Bacillus licheniformis and the impact on the secretion capability

Paper ID Volume ID Publish Year Pages File Format Full-Text
25302 43566 2007 9 PDF Available
Title
Targeted deletion of genes encoding extracellular enzymes in Bacillus licheniformis and the impact on the secretion capability
Abstract

The general secretory pathway is routinely concerned with a multitude of extracellular enzymes. By eliminating obstructive competitors the export machinery may transport larger quantities of remaining proteins under circumstances in which the secretion machinery is fully loaded. Hence, in this study, genes encoding efficiently expressed but dispensable exoenzymes were knocked out in Bacillus licheniformis MD1. Single, double, and triple mutants with deletions of celA, chiA, and amyB, respectively, were generated via in vivo recombination by making use of a vector with a temperature sensitive origin of replication.Overexpression of a heterologous amylase gene on a multi-copy plasmid, a common scenario in biotechnological processes, resulted in an articulate reduction of chromosomally encoded extracellular enzyme activities indicating that the secretion machinery works to capacity in such transformants. Deletion mutants with the expression plasmid displayed enhanced amylase activities compared to the strain with the wild type genetic background. In addition, the chromosomally encoded protease activity was clearly higher in transformants with deletions.

Keywords
Bacillus licheniformis; Amylase; Chitinase; Cellulase; Targeted deletion; Relief of bottlenecks; Extracellular enzymes
First Page Preview
Targeted deletion of genes encoding extracellular enzymes in Bacillus licheniformis and the impact on the secretion capability
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 130, Issue 2, 15 June 2007, Pages 124–132
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us