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Evaluation of peanut genotypes for in vitro plant regeneration using thidiazuron

Paper ID Volume ID Publish Year Pages File Format Full-Text
25311 43566 2007 6 PDF Available
Title
Evaluation of peanut genotypes for in vitro plant regeneration using thidiazuron
Abstract

A major limitation with the available protocols for in vitro regeneration of peanut (Arachis hypogaea L.) is their narrow application to very few select genotypes. Here, we report a protocol that can be applied across a broad spectrum of peanut market types, explant types and geographic regions using thidiazuron (TDZ). The effect of the timing of TDZ application to the culturing of both zygotic embryos and subsequent plantlet explants on MS medium is also reported. An extended use of TDZ and at a higher concentration (30 m/l) resulted in the greatest explant shoot average (∼13). However, a limited application of TDZ (10 d) was sufficient to induce shoot formation in peanut. Hypocotyl was the best explant type that induced the greatest shoot average (15) across market types followed by lamina (7.4). Spanish and Valencia were the most efficient market groups that induced shoots across explant types, consistently.

Keywords
Peanut regeneration; Peanut tissue culture; In vitro shoot formation; TDZ
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Evaluation of peanut genotypes for in vitro plant regeneration using thidiazuron
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 130, Issue 2, 15 June 2007, Pages 202–207
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us