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An siRNA-based system for differential regulation of ectopic gene expression constructs

Paper ID Volume ID Publish Year Pages File Format Full-Text
25317 43567 2007 8 PDF Available
Title
An siRNA-based system for differential regulation of ectopic gene expression constructs
Abstract

We describe an siRNA (short interfering RNA)-based approach for temporary and reversible regulation of engineered expression constructs in cells. Control of cloned genes can be achieved by cotransfection of unique siRNAs, complementary to artificial target sequences, which are integral parts of an expression vector. Application of this method allows simultaneous or mutual-differential regulation of two or more gene constructs within the same cell, reducing unwanted side effects. This method provides several advantages over promoter regulatory systems employing chemical compounds.

Keywords
RNAi; siRNA; Gene silencing; Gene regulation; Expression; Promoter regulation
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An siRNA-based system for differential regulation of ectopic gene expression constructs
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 128, Issue 4, 10 March 2007, Pages 762–769
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us