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Highly efficient gene targeting in the Aspergillus niger kusA mutant

Paper ID Volume ID Publish Year Pages File Format Full-Text
25318 43567 2007 6 PDF Available
Title
Highly efficient gene targeting in the Aspergillus niger kusA mutant
Abstract

Gene targeting frequencies in Aspergillus niger are often very low and hamper efficient functional genomics in this biotechnologically important fungus. Deletion of the A. niger kusA gene encoding the ortholog of the Ku70 protein in other eukaryotes, dramatically improved homologous integration efficiency and reached more than 80% compared to 7% in the wild-type background, when 500 bp homologous flanks were used. Furthermore, the use of the ΔkusA strain resulted in a high frequency of heterokaryon formation (70%) in primary transformants in the case disrupting an essential gene. Deletion of kusA had no obvious effect on the growth of the fungus, but renders the ΔkusA strain 10 times more sensitive to X-ray irradiation and two to three times more sensitive to UV exposure. The highly efficient gene targeting in combination with the A. niger genome sequence allows a systematic approach to generate gene knockouts and will help in improving the capacities of A. niger as producer of commercially interesting proteins and metabolites.

Keywords
Filamentous fungi; Functional genomics; Gene targeting; ku70; Gene knock outs; Strain improvement
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Highly efficient gene targeting in the Aspergillus niger kusA mutant
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 128, Issue 4, 10 March 2007, Pages 770–775
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us