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Fractionation of proteins with two-sided electro-ultrafiltration

Paper ID Volume ID Publish Year Pages File Format Full-Text
25329 43567 2007 13 PDF Available
Title
Fractionation of proteins with two-sided electro-ultrafiltration
Abstract

Downstream processing is a major challenge in bioprocess industry due to the high complexity of bio-suspensions itself, the low concentration of the product and the stress sensitivity of the valuable target molecules. A multitude of unit operations have to be joined together to achieve an acceptable purity and concentration of the product. Since each of the unit operations leads to a certain product loss, one important aim in downstream-research is the combination of different separation principles into one unit operation. In the current work a dead-end membrane process is combined with an electrophoresis operation. In the past this concept has proven successfully for the concentration of biopolymers. The present work shows that using different ultrafiltration membranes in a two-sided electro-filter apparatus with flushed electrodes brought significant enhancement of the protein fractionation process. Due to electrophoretic effects, the filtration velocity could be kept on a very high level for a long time, furthermore, the selectivity of a binary separation process carried out exemplarily for bovine serum albumin (BSA) and lysozyme (LZ) could be greatly increased; in the current case up to a value of more than 800.Thus the new two-sided electro-ultrafiltration technique achieves both high product purity and short separation times.

Keywords
BSA, bovine serum albumin; LZ, chicken egg lysozyme; PAN, polyacrylonitrile; PES, polyethersulfone; PWF, pure water flux; SEC, size exclusion chromatography; HPLC, high performance liquid chromatography; MWCO, molecular weight cut offElectrofiltration; Ul
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 128, Issue 4, 10 March 2007, Pages 895–907
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us