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Preparation of liposomal gene therapy vectors by a scalable method without using volatile solvents or detergents

Paper ID Volume ID Publish Year Pages File Format Full-Text
25358 43570 2007 10 PDF Available
Title
Preparation of liposomal gene therapy vectors by a scalable method without using volatile solvents or detergents
Abstract

A scalable and safe method was developed to prepare liposomal carriers for entrapment and delivery of genetic material. The carrier systems were composed of endogenously occurring dipalmitoylphosphatidylcholine (DPPC), negatively charged dicetylphosphate (DCP), cholesterol (CHOL) and glycerol (3%, v/v). Liposomes were prepared by a modified and improved version of the heating method in which no harmful chemical or procedure is involved. Anionic lipoplexes were formed by incorporating plasmid DNA (pCMV-GFP) to the liposomes by the mediation of calcium ions. Transfection efficiency and toxicity of the lipoplexes were evaluated in CHO-K1 cells using flow cytometry and MTT assay, respectively. Controls included DNA-Ca2+ complexes (without lipids), anionic liposome–DNA complexes (with no Ca2+), and a commercially available cationic liposomal formulation. Results indicated fast and reproducible formation of non-toxic lipoplexes that possess long-term stability, high DNA entrapment capacity (81%) and high transfection efficiency. The lipoplex preparation method has the potential of large-scale manufacture of safe and efficient carriers of nucleic acid drugs.

Keywords
Anionic liposomes; Calcium; DNA; Gene transfer; Heating method
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Preparation of liposomal gene therapy vectors by a scalable method without using volatile solvents or detergents
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 129, Issue 4, 10 May 2007, Pages 604–613
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us