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Monoclonal antibody against non-dominant epitopes of HBV e Ag was raised by antigen–antibody co-immunization

Paper ID Volume ID Publish Year Pages File Format Full-Text
25360 43570 2007 8 PDF Available
Title
Monoclonal antibody against non-dominant epitopes of HBV e Ag was raised by antigen–antibody co-immunization
Abstract

Detection of hepatitis B e antigen (HBeAg) in the sera of individuals infected with hepatitis B virus (HBV) can indicate both a high infectivity of the disease and a poor prognosis of disease treatment. Most of monoclonal antibodies raised against HBV e proteins interact with immuno-dominant epitopes, such as HBeAg-β. In order to raise antibodies against non-dominant epitopes of HBV e protein, in this study, mice were immunized with both recombinant HBeAg (rHBeAg) and an anti-HBeAg antibody (EWB) recognizing a dominant antigenic epitope of HBeAg (HBeAg-β epitope). With this strategy, we successfully selected two monoclonal antibodies, S-29-3 and S-72-3. Both S-29-3 and S-72-3 bind to recombinant HBeAg with a high affinity. The epitope mapping assay determined that the S-73-2 recognizes the N-terminal of HBeAg (1–118 aa) and the S-29-3 recognizes the C-terminal of HBeAg (91–149 aa). Further experiment showed that these two antibodies could be formed a pair-Abs that is used in detecting native HBeAg from the sera of HBV patients. The conclusion is that the developed method is useful to raise mAb against non-dominant epitopes in given Ag.

Keywords
HBV, hepatitis B virus; HBeAg, hepatitis B e antigen; aa, amino acid; HRP, horseradish peroxidase; BSA, bovine serum albumin; ELISA, enzyme-linked immunosorbent assayHepatitis B virus (HBV); HBeAg; Co-immunization; Epitope; Anti-HBeAg immunoassay; ELISA
First Page Preview
Monoclonal antibody against non-dominant epitopes of HBV e Ag was raised by antigen–antibody co-immunization
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 129, Issue 4, 10 May 2007, Pages 620–627
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering