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Enhanced production of secretory β1,3-N-acetylglucosaminyltransferase 2 fusion protein into hemolymph of Bombyx mori larvae using recombinant BmNPV bacmid integrated signal sequence

Paper ID Volume ID Publish Year Pages File Format Full-Text
25368 43570 2007 8 PDF Available
Title
Enhanced production of secretory β1,3-N-acetylglucosaminyltransferase 2 fusion protein into hemolymph of Bombyx mori larvae using recombinant BmNPV bacmid integrated signal sequence
Abstract

The enhanced secretion of β1,3-N-acetylglucosaminyltransferase 2 (β3GnT2) fusion protein into the hemolymph of Bombyx mori larvae was studied using a recombinant B. mori nucleopolyhedrovirus (BmNPV) bacmid integrating seven signal sequences. When the BmNPV bacmid encoding the signal sequences from the silkworm B. mori bombyxin (bx) and B. mori prophenoloxidase-activating enzyme (ppae) was injected into silkworm larvae, 56.1 and 51.5 mU/ml β3GnT, respectively, were secreted into the hemolymph of silkworm larvae. For bx, 97.3% of the total β3GnT activity was secreted into hemolymph, and only 1.1% remained in the intestines of silkworm larvae. For ppae, 90.8% of the total β3GnT activity was secreted to the hemolymph, but 7.8% remained in the intestines of silkworm larvae. Using the BmNPV bacmid encoding bx, the amount of secreted β3GnT was 91 μg per larva, which was 2.5% of the total amount of protein in the hemolymph.

Keywords
Bombyx mori nucleopolyhedrovirus; Bacmid; Bombyx mori; Signal peptide; β1,3-N-acetylglucosaminyltransferaseAcNPV, Autographa californica nuclear polyhedrosis; Bacmid, baculovirus shuttle vector; BES, baculovirus expression system; BmNPV, Bombyx mori nucle
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Enhanced production of secretory β1,3-N-acetylglucosaminyltransferase 2 fusion protein into hemolymph of Bombyx mori larvae using recombinant BmNPV bacmid integrated signal sequence
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 129, Issue 4, 10 May 2007, Pages 681–688
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us