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Improving the nutritive value of tubers: Elevation of cysteine and glutathione contents in the potato cultivar White Lady by marker-free transformation

Paper ID Volume ID Publish Year Pages File Format Full-Text
25386 43571 2007 9 PDF Available
Title
Improving the nutritive value of tubers: Elevation of cysteine and glutathione contents in the potato cultivar White Lady by marker-free transformation
Abstract

The amino acids that limit the nutritive value of potato are the sulfur containing amino acids methionine and cysteine. Manipulation of the targeted amino acid biosynthesis is a way to circumvent this problem. Cysteine is synthesised from O-acetyl-l-serine formed by serine acetyltransferase (SAT). To increase the cysteine content of the commercial potato cultivar White Lady the chimeric SAT-coding cysE gene from Escherichia coli under the control of the constitutive CaMV 35S promoter and fused to the chloroplast targeting rbcS 5′-transit peptide sequence was introduced into the White Lady genome. Novelty of the approach was the application of marker-free transformation. Two transgenic lines were obtained that accumulated the cysE mRNA in high amounts. Crude leaf extracts of these plants exhibited up to 80- and 20-fold higher SAT activity in leaves and tubers, respectively, than those prepared from non-transformed plants. Levels of cysteine and glutathione both in leaves and tubers were 1.5-fold higher in average than in control plants. The alterations observed had no effect on tuber yield and sprouting behaviour. Gas chromatography coupled to mass spectrometry showed that all other amino acids than cysteine were unaffected. Here we demonstrate for the first time that the cysteine content of tubers can be enhanced by metabolic engineering.

Keywords
OAS, O-acetyl-l-serine; OAS-TL, O-acetylserine(thiol)lyase; SAT, serine acetyltransferaseCysteine; Glutathione; Metabolite analysis; Serine acetyltransferase; Solanum tuberosum; Transgenic plants
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Improving the nutritive value of tubers: Elevation of cysteine and glutathione contents in the potato cultivar White Lady by marker-free transformation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 128, Issue 2, 1 February 2007, Pages 335–343
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us