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Enhanced activity of 3α-hydroxysteroid dehydrogenase by addition of the co-solvent 1-butyl-3-methylimidazolium (l)-lactate in aqueous phase of biphasic systems for reductive production of steroids

Paper ID Volume ID Publish Year Pages File Format Full-Text
25390 43571 2007 7 PDF Available
Title
Enhanced activity of 3α-hydroxysteroid dehydrogenase by addition of the co-solvent 1-butyl-3-methylimidazolium (l)-lactate in aqueous phase of biphasic systems for reductive production of steroids
Abstract

The enzyme activity of 3α-hydrosteroid dehydrogenase (HSDH) was enhanced by the addition of the co-solvent 1-butyl-3-methylimidazolium (l)-lactate ([Bmim][lactate]) to 50 mM Tris–HCl buffer. When utilizing [Bmim][lactate], the reaction velocity of HSDH increased. Also, reductive production of androsterone was investigated in an aqueous–organic solvent biphasic system containing 5% [Bmim][lactate] as the co-solvent of aqueous phase. In a coupled-enzyme system comprising HSDH and formate dehydrogenase (FDH), a two-fold increase in production rate of androsterone was obtained when utilizing [Bmim][lactate] with NADH regeneration.

Keywords
HSDH, 3α-hydrosteroid dehydrogenase; FDH, formate dehydrogenase; [Emim][CF3SO3], 1-ethyl-3-methylimidazolium trifluoromethanesulfonate; [Bmim][CF3SO3], 1-butyl-3-methylimidazolium trifluoromethanesulfonate; [Bmim][lactate], 1-butyl-3-methylimidazolium lac
First Page Preview
Enhanced activity of 3α-hydroxysteroid dehydrogenase by addition of the co-solvent 1-butyl-3-methylimidazolium (l)-lactate in aqueous phase of biphasic systems for reductive production of steroids
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 128, Issue 2, 1 February 2007, Pages 376–382
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering