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Dithiocarbamate fungicide thiram detection: Comparison of bioluminescent and fluorescent whole-cell bioassays based on hsp22 stress promoter induction

Paper ID Volume ID Publish Year Pages File Format Full-Text
25404 43572 2006 11 PDF Available
Title
Dithiocarbamate fungicide thiram detection: Comparison of bioluminescent and fluorescent whole-cell bioassays based on hsp22 stress promoter induction
Abstract

Detection of toxic substances interfering with endocrine system is one of the major preoccupations of the European community. A whole-cell bioassay for pollution detection based on stress induction has been designed. Well characterized toxicants, cadmium chloride and thiram (a dithiocarbamate fungicide), were used to optimize the detection conditions such as time-course conditions, cell line and reporter gene to be used. HeLa cells containing the firefly luciferase (luc) reporter gene under the control of the Drosophila melanogaster hsp22 promoter were compared to liver cells (HepG2) containing the same stress gene promoter fused either to the luc or the EGFP (Enhanced-Green Fluorescent Protein) gene. The sensitivity of the obtained bioassay was found to be enhanced by the concomitant use of liver cells and EGFP reporter gene. The detection limits of the toxicants were then lowered from 1 to 0.1 μM and from 1 to 0.01 μM for CdCl2 and thiram, respectively.

Keywords
Thiram; Cadmium; Firefly luciferase; EGFP; Whole-cell bioassay
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Dithiocarbamate fungicide thiram detection: Comparison of bioluminescent and fluorescent whole-cell bioassays based on hsp22 stress promoter induction
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 124, Issue 2, 13 July 2006, Pages 392–402
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us