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Production of xylitol by metabolically engineered strains of Bacillus subtilis

Paper ID Volume ID Publish Year Pages File Format Full-Text
25414 43573 2007 8 PDF Available
Title
Production of xylitol by metabolically engineered strains of Bacillus subtilis
Abstract

Xylitol-phosphate dehydrogenase (XPDH) genes from several Gram-positive bacteria were isolated and expressed in Bacillus subtilis. The substrate specificities of the recombinant XPDH enzymes were compared and it was found that the XPDH enzymes of Lactobacillus rhamnosus and Clostridium difficile had the highest selectivity towards d-xylulose 5-phosphate. Expression of these two XPDH enzymes in d-ribulose and d-xylulose producing B. subtilis strain resulted in strains of B. subtilis capable of converting d-glucose into xylitol at around 23% yield.

Keywords
Xylitol; Xylitol-phosphate dehydrogenase; Bacillus subtilis
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Production of xylitol by metabolically engineered strains of Bacillus subtilis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 128, Issue 1, 30 January 2007, Pages 24–31
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us