fulltext.study @t Gmail

A proportional analysis method using non-kinetic real-time PCR

Paper ID Volume ID Publish Year Pages File Format Full-Text
25416 43573 2007 9 PDF Available
Title
A proportional analysis method using non-kinetic real-time PCR
Abstract

Prompted by increasing interest in proportional analysis of genetic types, we developed a simple assay technique for determining the ratio of a specific target gene in the total genes that can be amplified with the same PCR primer. The key feature of this method is that the following two tasks are performed in a single-tube real-time PCR system: task 1, PCR amplification of the total genes including the target using a labeled PCR primer, with concurrent monitoring of the total copy number of the PCR product; task 2, detection of the signal of the target gene at each cycle of amplification, using a labeled nucleotide probe. In principle, the ratio of the target gene to the total genes is represented by the signal detected in ‘task 2’ at the cycle in which the PCR product reached a prescribed copy number (assessed by ‘task 1’).

Keywords
BODIPY FL, 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionic acid; bps, base pairs; dATP, 2′-deoxyadenosine-5′-triphosphate; dCTP, 2′-deoxycytidine-5′-triphosphate; dGTP, 2′-deoxyguanosine-5′-triphosphate; DNA, deoxyribonucleic acid; dUT
First Page Preview
A proportional analysis method using non-kinetic real-time PCR
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 128, Issue 1, 30 January 2007, Pages 41–49
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us