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Electrochemical biosensor for catechol using agarose–guar gum entrapped tyrosinase

Paper ID Volume ID Publish Year Pages File Format Full-Text
25420 43573 2007 6 PDF Available
Title
Electrochemical biosensor for catechol using agarose–guar gum entrapped tyrosinase
Abstract

An electrochemical biosensor using tyrosinase was constructed for the determination of catechol. The enzyme was extracted from a plant source Amorphophallus companulatus and entrapped in agarose–guar gum composite biopolymer matrix. Catechol was determined by direct reduction of biocatalytically liberated quinone species at −0.1 V versus Ag/AgCl (3 M KCl). The response was found to be linear and concentration dependent in the range of 6 × 10−5 to 8 × 10−4 M with a lower detection limit of 6 μM. It has reusability up to 20 cycles and a shelf life of more than 2 months when stored at 4 °C.

Keywords
Tyrosinase; Catechol; Biopolymers; Entrapment; Electrochemical sensor
First Page Preview
Electrochemical biosensor for catechol using agarose–guar gum entrapped tyrosinase
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 128, Issue 1, 30 January 2007, Pages 80–85
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering