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Stabilization of interface-binding chloroperoxidase for interfacial biotransformation

Paper ID Volume ID Publish Year Pages File Format Full-Text
25421 43573 2007 7 PDF Available
Title
Stabilization of interface-binding chloroperoxidase for interfacial biotransformation
Abstract

The stability of an interface-binding chloroperoxidase (CPO) against the deactivation effect of H2O2 was examined. Native CPO was conjugated with polystyrene and thus self-assembled at the water–oil interface. Although the interface-assembled CPO showed improved stability as compared to native CPO, enzyme deactivation as a result of the side effect of H2O2, still limits the overall productivity of the enzyme. Two approaches to further improve the stability of CPO were examined in this work. In one approach, several stabilizers including poly(ethylene glycol) (PEG), PEI, glycerol, sugars and sucrose monododecanoate were used; while in a second approach, in situ generation of hydrogen peroxide (H2O2) by using glucose oxidase (GOx) was applied. PEG was found exceptional in that it increased both the operational and storage stability of CPO. The best improvement of enzyme productivity was obtained with addition of PEG which led to an increase of 57% for interface-bound CPO and 33% for native CPO. One interesting observation with PEI is that it enhanced the storage stability against H2O2 deactivation, but did not affect the enzyme's operational stability. On the other hand, glucose enhanced the operational stability by two folds, but exhibited no significant effect on storage stability. It was also found that the extended operational lifetime of CPO with in situ generation of H2O2 by GOx was a result that combines the stabilizing effect of glucose and lowered concentration of H2O2. Interestingly, the addition of stabilizers could improve the enantioselectivity of CPO by as much as 10%.

Keywords
Chloroperoxidase; Interfacial biocatalysis; Epoxidation; Enzyme stability; Polymer–enzyme conjugate
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Stabilization of interface-binding chloroperoxidase for interfacial biotransformation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 128, Issue 1, 30 January 2007, Pages 86–92
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us