fulltext.study @t Gmail

Phenotypes and fed-batch fermentation of ubiquinone-overproducing fission yeast using ppt1 gene

Paper ID Volume ID Publish Year Pages File Format Full-Text
25424 43573 2007 12 PDF Available
Title
Phenotypes and fed-batch fermentation of ubiquinone-overproducing fission yeast using ppt1 gene
Abstract

Ubiquinone (UQ), a component of the electron transfer system in many organisms, has been widely used for pharmaceuticals and cosmetics. In this study, we cloned and overexpressed the full-length ppt1 (MTppt1) gene, which encodes p-hydroxybenzoate:polyprenyltransferase and ERppt1 gene, which was modified to be localized on endoplasmic reticulum in fission yeast. The yeast MTppt1 and ERppt1 transgenic lines showed about 3.7 and 5.1 times increment in UQ content and the recombinant yeasts with a higher UQ level are more resistant to H2O2, Cu2+ and NaCl, and interestingly their growth was also faster than the wild type at lower temperature. For large-scale cultivation, the direct feedback control of glucose using an on-line ethanol concentration monitor for ubiquinone production of yeast ERppt1 by high-cell-density fermentation was investigated and the fermentation parameters (e.g., dissolved oxygen, pH, ethanol concentration, oxygen uptake rate, carbon dioxide evolution rate and respiration quotient) were also discussed. After 90 h cultures, the yeast dry cell weight reached 57 g l−1 and the ubiquinone yield reached 23 mg l−1. In addition, plasmid stability was maintained at high level throughout the fermentation.

Keywords
Cloning; Subcellular localization; Fission yeast; Oxidative stress; Fed-batch fermentation; Ubiquinone-10
First Page Preview
Phenotypes and fed-batch fermentation of ubiquinone-overproducing fission yeast using ppt1 gene
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 128, Issue 1, 30 January 2007, Pages 120–131
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us