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Gene silencing through RNA interference (RNAi) in vivo: Strategies based on the direct application of siRNAs

Paper ID Volume ID Publish Year Pages File Format Full-Text
25434 43574 2006 14 PDF Available
Title
Gene silencing through RNA interference (RNAi) in vivo: Strategies based on the direct application of siRNAs
Abstract

RNA interference (RNAi) offers great potential not only for in vitro target validation, but also as a novel therapeutic strategy based on the highly specific and efficient silencing of a target gene, e.g. in tumor therapy. Since it relies on small interfering RNAs (siRNAs), which are the mediators of RNAi-induced specific mRNA degradation, a major issue is the delivery of therapeutically active siRNAs into the target tissue/target cells in vivo. For safety reasons, strategies based on (viral) vector delivery may be of only limited clinical use. The more desirable approach is to directly apply catalytically active siRNAs. This review highlights the recent knowledge on the guidelines for the selection of siRNAs which show high activity in the absence of non-specific siRNA effects. It then focuses on approaches to directly use siRNA molecules in vivo and gives a comprehensive overview of in vivo studies based on the direct application of siRNAs to induce RNAi. One promising approach is the in vivo siRNA delivery through complexation of chemically unmodified siRNAs with polyethylenimine (PEI). The anti-tumoral effects of PEI/siRNA-based targeting of tumor-relevant genes in vivo are described.

Keywords
RNAi; siRNAs; In vivo siRNA delivery; Polyethylenimine; PEI; Gene-silencingGFP, green fluorescent protein; HER-2, human epidermal growth factor receptor-2; PEI, polyethylenimine; PTN, pleiotrophin; RISC, RNA-induced silencing complex; RNAi, RNA interferen
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Gene silencing through RNA interference (RNAi) in vivo: Strategies based on the direct application of siRNAs
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 124, Issue 1, 25 June 2006, Pages 12–25
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us