fulltext.study @t Gmail

Water immiscible ionic liquids as solvents for whole cell biocatalysis

Paper ID Volume ID Publish Year Pages File Format Full-Text
25446 43574 2006 9 PDF Available
Title
Water immiscible ionic liquids as solvents for whole cell biocatalysis
Abstract

Whole cell biocatalysis can effectively be used for the production of enantiomerically pure compounds, but efficiency is often low. Toxicity and poor solubility of substrates and products are the main obstacles. In this study, water immiscible ionic liquids are shown to have no damaging effects on the cell membranes of Escherichia coli and Saccharomyces cerevisiae. Thus, they can be used as biocompatible solvents for microbial biotransformations exemplified by an increase in yield of chiral alcohol synthesis. As key point to the success of these processes, the distribution ratio of the reactants between the ionic liquid and the aqueous phase was identified. The use of ionic liquids as substrate reservoir and in situ extracting agent for the asymmetric reduction of various ketones resulted in an increase of chemical yield from <50% to 80–90% in simple batch processes. (R)-1-(4-chlorophenyl)ethanol was produced at a higher initial reaction rate in the biphasic system (>50 μM s−1 L−1) compared to the aqueous system. This result demonstrates that good mass transfer rates can be obtained despite the relatively high viscosity of ionic liquids.

Keywords
Ionic liquid; Biocatalysis; Whole cell; Multiphase system; Asymmetric reduction; Chiral alcohol
First Page Preview
Water immiscible ionic liquids as solvents for whole cell biocatalysis
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 124, Issue 1, 25 June 2006, Pages 182–190
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us