fulltext.study @t Gmail

Phosphorylase coupling as a tool to convert cellobiose into amylose

Paper ID Volume ID Publish Year Pages File Format Full-Text
25489 43576 2007 7 PDF Available
Title
Phosphorylase coupling as a tool to convert cellobiose into amylose
Abstract

This work aims to establish the enzymatic process to produce amylose from cellobiose. Incubation of cellobiose with cellobiose phosphorylase and α-glucan phosphorylase in the presence of maltotetraose and a catalytic amount of inorganic phosphate at 45 °C for 16 h resulted in the production of linear α-1,4-glucan with a 19.3% (w/v, against cellobiose weight) yield. The yield was successfully improved (32.4%) when mutarotase and glucose oxidase were added to remove glucose in the reaction mixture. The weight-average molecular weight of the product was precisely controlled from 42 to 720 kDa by changing the initial molar ratio of cellobiose to maltotetraose. The combined use of two different phosphorylases should be a useful tool in converting β-1,4-linked-polysaccharide into α-1,4-linked-polysaccharide.

Keywords
Cellobiose; Amylose; Conversion; Cellobiose phosphorylase; α-Glucan phosphorylase
First Page Preview
Phosphorylase coupling as a tool to convert cellobiose into amylose
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 127, Issue 3, 10 January 2007, Pages 496–502
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us