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Effect of nitrogen limitation on the ergosterol production by fed-batch culture of Saccharomyces cerevisiae

Paper ID Volume ID Publish Year Pages File Format Full-Text
25496 43577 2006 8 PDF Available
Title
Effect of nitrogen limitation on the ergosterol production by fed-batch culture of Saccharomyces cerevisiae
Abstract

The diversity and content of available nitrogen sources in the growth medium both are very important in the accumulation of ergosterol in the yeast cell membrane. Growth on the good nitrogen sources such as ammonia can harvest more yeast cells than on poor ones, but ergosterol content in those yeast cells is relatively lower. Ergosterol content, one of the most variable parameters in ergosterol production by yeast cultivation, is greatly influenced by nitrogen limitation. The aim of our work was to study how the nitrogen sources affected the membrane ergosterol content and increase the total ergosterol yield. On the premise of keeping high ergosterol content in yeast cell, the ergosterol yield was enhanced by increasing the yeast biomass. Direct feed back control of glucose using an on-line ethanol concentration monitor was introduced to achieve high cell density. Ammonia, which acted as nitrogen source, was added to adjust pH during fermentation process, but its addition needed careful control. Cultivation in 5 L bioreactor was carried out under following conditions: culture temperature 30 ± 1 °C, pH 5.5 ± 0.1, agitation speed 600 rpm, controlling ethanol concentration bellow 1% and controlling ammonium ion concentration bellow 0.1 mol/L. Under these conditions the yeast dry weight reached 95.0 ± 2.6 g/L and the ergosterol yield reached 1981 ± 34 mg/L.

Keywords
Ergosterol; Saccharomyces cerevisiae; Nitrogen; Fed-batch culture; High cell density
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Effect of nitrogen limitation on the ergosterol production by fed-batch culture of Saccharomyces cerevisiae
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 122, Issue 3, 10 April 2006, Pages 285–292
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us