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Synthesis of the food flavoring methyl benzoate by genetically engineered Saccharomyces cerevisiae

Paper ID Volume ID Publish Year Pages File Format Full-Text
25498 43577 2006 9 PDF Available
Title
Synthesis of the food flavoring methyl benzoate by genetically engineered Saccharomyces cerevisiae
Abstract

Current means of production for plant-derived aroma compounds include chemical synthesis and extraction from plant material. Both methods are environmentally detrimental and relatively expensive: plant material is only seasonally available and only a small subset of the plant biomass produces the desired aroma compounds, while organic synthesis inevitably involves waste byproducts with a negative ecological impact. Benzenoids are a class of plant metabolites that includes a number of aroma compounds. This paper explores, for the first time, the feasibility of producing benzenoids in yeast. We present a method for the production of the phenylpropanoid methyl benzoate in Saccharomyces cerevisiae using benzoic acid as a substrate, by heterologous expression of Antirrhinum majus benzoic acid methyl transferase. Production was pH dependent with a maximal yield of approximately 50 μg of methyl benzoate per liter of culture per hour, and with linear kinetics over at least 24 h. In addition, we have analyzed two alternative expression vectors for the production of benzoic acid methyl transferase in S. cerevisiae: a constitutive triosephosphate isomerase promoter-based system was compared with a copper-inducible CUP1 promoter system. We find major differences in the amounts of methylbenzoate produced by these respective systems. Potential applications are discussed.

Keywords
Saccharomyces cerevisiae; Methyl benzoate; Benzoic acid; Biotransformation; Aroma; Benzoic acid methyltransferase
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Synthesis of the food flavoring methyl benzoate by genetically engineered Saccharomyces cerevisiae
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 122, Issue 3, 10 April 2006, Pages 307–315
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us