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Recombinant human erythropoietin produced in milk of transgenic pigs ☆

Paper ID Volume ID Publish Year Pages File Format Full-Text
25503 43577 2006 10 PDF Available
Title
Recombinant human erythropoietin produced in milk of transgenic pigs ☆
Abstract

We have developed a line of transgenic swine harboring recombinant human erythropoietin through microinjection into fertilized one cell pig zygotes. Milk from generations F1 and F2 transgenic females was analyzed, and hEPO was detected in milk from all lactating females at concentrations of approximately 877.9 ± 92.8 IU/1 ml. The amino acid sequence of rhEPO protein in the transgenic pig milk matched that of commercial rhEPO produced from cultured animal cells. In addition, an F-36 cell line, which proliferates in the presence of hEPO or commercial EPO, was induced to synthesize erythroid by extracts from tg sow milk. This study provides evidence that production of purified rhEPO from transgenic pig milk is a potentially valuable technology, and can be used as a cost-effective alternative in clinical applications as well as providing other clinical advantages.

Keywords
mWAP, mouse whey acidic protein; rhEPO, recombinant human erythropoietinErythropoietin; Transgenic animals; Microinjection; Mammary gland; Expression
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Recombinant human erythropoietin produced in milk of transgenic pigs ☆
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 122, Issue 3, 10 April 2006, Pages 362–371
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us