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Polyketone polymer: A new support for direct enzyme immobilization

Paper ID Volume ID Publish Year Pages File Format Full-Text
25517 43578 2007 9 PDF Available
Title
Polyketone polymer: A new support for direct enzyme immobilization
Abstract

Polyketone polymer [COCH2CH2]n, obtained by copolymerization of ethene and carbon monoxide, is utilized for immobilization of three different enzymes, one peroxidase from horseradish (HRP) and two amine oxidases, from bovine serum (BSAO) and lentil seedlings (LSAO). The easy immobilization procedure is carried out in diluted buffer, at pH 7.0 and 3 °C, gently mixing the proteins with the polymer. No bifunctional reagents and spacer arms are required for the immobilization, which occurs exclusively via a large number of hydrogen bonds between the carbonyl groups of the polymer and the NH groups of the polypeptidic chain. Experiments demonstrate a high linking capacity of polymer for BSAO and an extraordinary strong linkage for LSAO. Moreover, activity measurements demonstrate that immobilized LSAO totally retains the catalytic characteristics of the free enzyme, where only a limited increase of KM value is observed. Finally, the HRP-activated polymer is successfully used as active packed bed of an enzymatic reactor for continuous flow conversion and flow injection analysis of hydrogen peroxide containing solutions.

Keywords
AO, copper/TPQ containing amine oxidase; BSAO, bovine serum amine oxidase; dppp; 1,3-bis(diphenylphosphino)propane; LSAO, lentil seedling amine oxidase; HRP, horseradish peroxidaseImmobilized enzymes; Polyketone polymer; Hydrogen bonds; F.I.A.; Copper con
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Polyketone polymer: A new support for direct enzyme immobilization
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 127, Issue 4, 20 January 2007, Pages 670–678
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
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Price after discount Only $4.95
100% Money Back Guarantee
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