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Structural aspects of the Mucor bacilliformis proteinase, a new member of the aspartyl-proteinase family

Paper ID Volume ID Publish Year Pages File Format Full-Text
25531 43579 2006 10 PDF Available
Title
Structural aspects of the Mucor bacilliformis proteinase, a new member of the aspartyl-proteinase family
Abstract

Bovine chymosin is considered the best milk-clotting enzyme for cheese manufacture; however, the thermophilic Mucor pusillus proteinase is also used nowadays. We herein report structural aspects of the aspartyl proteinase from the local mesophilic Mucor bacilliformis strain. Sequence data indicate a high similarity degree to those of other family members. The protein is monomeric, not glycosylated, has two disulfide bridges, and mainly includes beta structure. A molecular model was built by using the Rhizopus chinensis proteinase structure as the template. Sequence analysis and comparison of our model with bovine chymosin and M. pusillus proteinase structures, indicate that the M. bacilliformis proteinase is at a similar evolutionary distance on a sequence level; as regards tertiary structure, the M. bacilliformis proteinase superimposes on the bovine chymosin structure in a fashion similar to that of the M. pusillus proteinase. Overall results suggest that this novel proteinase can be utilized as a good milk-clotting enzyme in the dairy industry.

Keywords
ACN, acetonitrile; CNBr, cyanogen bromide; CNBr/KI, cyanogen bromide/potassium iodide; PVDF, polyvinylidene difluoride; 4-VP, 4-vinlpyridine; TFA, trifluoracetic acidMucor bacilliformis proteinase; Aspartyl-proteinase family; Mesophilic proteinases; Chees
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Structural aspects of the Mucor bacilliformis proteinase, a new member of the aspartyl-proteinase family
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 123, Issue 4, 10 June 2006, Pages 443–452
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us