fulltext.study @t Gmail

High efficient degradation of dyes with lignin peroxidase coupled with glucose oxidase

Paper ID Volume ID Publish Year Pages File Format Full-Text
25535 43579 2006 8 PDF Available
Title
High efficient degradation of dyes with lignin peroxidase coupled with glucose oxidase
Abstract

The H2O2 supply strategy was one of crucial factors for high efficient degradation of pollutants with lignin peroxidase (LiP). In this paper, an attempt was made to couple a H2O2 producing enzymatic reaction to the LiP catalyzed oxidation of dyes. H2O2 needed was generated by glucose oxidase (GOD) and its substrate glucose. The generation rate of H2O2 could be easily controlled by adjusting the pH of the degradation system and the amount of GOD added. Due to the controlled release of H2O2, a sustainable constant activity of LiP was observed. The inhibition of LiP by high level H2O2 supplied externally by a single addition at the beginning of the experiments could be avoided. Degradation of three dyes (xylene cyanol, fuchsine and rhodamine B) with LiP coupled with GOD indicated that the present H2O2 supply strategy was very effective for improvement of the efficiency of the decolourization of dyes.

Keywords
Lignin peroxidase; Dye decolourization; Glucose oxidase; Controlled release; Hydrogen peroxide; Coupled enzymatic reaction
First Page Preview
High efficient degradation of dyes with lignin peroxidase coupled with glucose oxidase
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 123, Issue 4, 10 June 2006, Pages 483–490
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering