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A simplified QTL mapping approach for screening and mapping of novel AFLP markers associated with beef marbling

Paper ID Volume ID Publish Year Pages File Format Full-Text
25538 43580 2007 11 PDF Available
Title
A simplified QTL mapping approach for screening and mapping of novel AFLP markers associated with beef marbling
Abstract

Genome screening of quantitative trait loci (QTL) for a complex trait is usually costly and highly laborious, as it requires a large number of markers spanning the whole genome. Here we present a simplified approach for screening and mapping of QTL-linked markers for beef marbling using a Wagyu × Limousin F2 reference population. This simplified approach involves integration of the amplified fragment length polymorphism (AFLP) with DNA pooling and selective genotyping and comparative bioinformatics tools. AFLP analysis on two high and two low marbling DNA pools yielded ten visually different markers. Among them, four were confirmed based on individual AFLP validation. Sequencing and in silico characterization assigned two of these AFLP markers to bovine chromosomes 1 (BTA1) and 13 (BTA13), which are orthologous to human chromosomes HSA21q22.2 and HSA10p11.23 with both regions harboring QTL for obesity-related phenotypes. Both AFLP markers showed significantly large additive genetic effects (0.28 ± 0.11 on BTA1 and 0.54 ± 0.21 on BTA13) on beef-marbling score (BMS) (P < 0.05). Overall, this approach is less time consuming, inexpensive and in particular, suitable for screening and mapping QTL-linked markers when targeting one or a few complex traits.

Keywords
QTL mapping; A simplified approach; AFLP; DNA pooling; Bioinformatics tools; Beef marbling
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A simplified QTL mapping approach for screening and mapping of novel AFLP markers associated with beef marbling
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 127, Issue 2, 1 January 2007, Pages 177–187
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us