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An effective cold inducible expression system developed in Pseudoalteromonas haloplanktis TAC125

Paper ID Volume ID Publish Year Pages File Format Full-Text
25540 43580 2007 12 PDF Available
Title
An effective cold inducible expression system developed in Pseudoalteromonas haloplanktis TAC125
Abstract

A regulative two-component system previously identified in Pseudoalteromonas haloplanktis TAC125 was used to construct a cold inducible expression system that is under the control of l-malate. Performances of the inducible system were tested for both psychrophilic and mesophilic protein production using two “difficult” proteins as control. The results obtained demonstrated that both psychrophilic β-galactosidase and yeast α-glucosidase are produced in a fully soluble and catalytically competent form. Optimal conditions for protein production, including growth temperature, growth medium and l-malate concentration were also investigated. Under optimized conditions yields of 620 and 27 mg/l were obtained for β-galactosidase and α-glucosidase, respectively.

Keywords
Psychrophiles; Protein production; Cold inducible system; β-Galactosidase; α-Glucosidase
First Page Preview
An effective cold inducible expression system developed in Pseudoalteromonas haloplanktis TAC125
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 127, Issue 2, 1 January 2007, Pages 199–210
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering