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Scale-up of recombinant Opc protein production in Escherichia coli for a meningococcal vaccine

Paper ID Volume ID Publish Year Pages File Format Full-Text
25564 43581 2006 6 PDF Available
Title
Scale-up of recombinant Opc protein production in Escherichia coli for a meningococcal vaccine
Abstract

Opc is an outer membrane protein from Neisseria meningitidis present in meningococcal vaccine preparations. The opc gene, codifying for this protein, was cloned in to Escherichia coli and the Opc protein was expressed under the control of a tryptophan promoter. The recombinant strain was grown in batch cultures. Opc was expressed as inclusion bodies at about 32% of the total cellular protein. We examined the scale-up culture conditions for the production of the recombinant Opc. The scale-up process was performed from 1.5 l to 50 l culture, using first, the constant power per unit of volume (P/V) as main scaling criteria, and then the oxygen mass transfer coefficient (KLa) scaling criteria to adjust the optimal aeration conditions. A final productivity of 52 mg l−1 h−1 was obtained at the 50 l culture scale compared with the 49 mg l−1 h−1 productivity at 1.5 l laboratory scale.

Keywords
Neisseria meningitides; Opc; Recombinant protein; Scale-up
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Scale-up of recombinant Opc protein production in Escherichia coli for a meningococcal vaccine
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 127, Issue 1, 15 December 2006, Pages 109–114
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us