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Use of whey protein beads as a new carrier system for recombinant yeasts in human digestive tract

Paper ID Volume ID Publish Year Pages File Format Full-Text
25568 43581 2006 10 PDF Available
Title
Use of whey protein beads as a new carrier system for recombinant yeasts in human digestive tract
Abstract

A new immobilizing protocol using whey protein isolates was developed to entrap recombinant Saccharomyces cerevisiae. The model yeast strain expresses the heterologous P45073A1 that converts trans-cinnamic acid into p-coumaric acid. Beads resulted from a cold-induced gelation of a whey protein solution (10%) containing yeasts (7.5 × 107 cells ml−1) into 0.1 M CaCl2. The viability and growth capability of yeasts were not altered by our entrapment process. The release and activity of immobilized yeasts were studied in simulated human gastric conditions. During the first 60 min of digestion, 2.2 ± 0.9% (n = 3) of initial entrapped yeasts were recovered in the gastric medium suggesting that beads should cross the gastric barrier in human. The P45073A1 activity of entrapped yeasts remained significantly higher (p < 0.05) than that of free ones throughout digestion (trans-cinnamic acid conversion rate of 63.4 ± 1.6% versus 51.5 ± 1.8% (n = 3) at 120 min). The protein matrix seemed to create a microenvironment favoring the activity of yeasts in the stringent gastric conditions. These results open up new opportunities for the development of drug delivery system using recombinant yeasts entrapped in whey protein beads. The main potential medical applications include biodetoxication or the correction of digestive enzyme deficiencies.

Keywords
Whey protein beads; Cold gelation; Recombinant Saccharomyces cerevisiae; Artificial stomach; Drug delivery system
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Use of whey protein beads as a new carrier system for recombinant yeasts in human digestive tract
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 127, Issue 1, 15 December 2006, Pages 151–160
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us