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Identification of low frequency knockout mutants in Dictyostelium discoideum created by single or double homologous recombination

Paper ID Volume ID Publish Year Pages File Format Full-Text
25572 43582 2006 4 PDF Available
Title
Identification of low frequency knockout mutants in Dictyostelium discoideum created by single or double homologous recombination
Abstract

Generation and characterization of knockout clones is a widely used approach to evaluate the specific function of a gene product in Dictyostelium discoideum. The mutant clones are generally obtained by double homologous recombination in the target gene. A frequent limitation to obtaining mutants is the low frequency of homologous recombination. Here we present an easy method to identify rare mutants, based on PCR analysis of pools of clones. This method also allows the isolation of functional knockout mutants created by a single homologous recombination event, which can be more frequent than a double recombination event.

Keywords
PCR; Mutant; Gene; Dictyostelium discoideum; Cloning; Homologous recombination
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Identification of low frequency knockout mutants in Dictyostelium discoideum created by single or double homologous recombination
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 122, Issue 1, 9 March 2006, Pages 1–4
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us