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Fractionation of follicle stimulating hormone charge isoforms in their native form by preparative electrophoresis technology

Paper ID Volume ID Publish Year Pages File Format Full-Text
25579 43582 2006 13 PDF Available
Title
Fractionation of follicle stimulating hormone charge isoforms in their native form by preparative electrophoresis technology
Abstract

Complex glycoprotein biopharmaceuticals, such as follicle stimulating hormone (FSH), erythropoietin and tissue plasminogen activator consist of a range of charge isoforms due to the extent of sialic acid capping of the glycoprotein glycans. Sialic acid occupies the terminal position on the oligosaccharide chain, masking the penultimate sugar residue, galactose from recognition and uptake by the hepatocyte asialoglycoprotein receptor. It is therefore well established that the more acidic charge isoforms of glycoprotein biopharmaceuticals have higher in vivo potencies than those of less acidic isoforms due to their longer serum half-life. Current strategies for manipulating glycoprotein charge isoform profile involve cell engineering or altering bioprocesss parameters to optimise expression of more acidic or basic isoforms, rather than downstream separation of isoforms. A method for the purification of a discrete range of bioactive recombinant human FSH (rhFSH) charge isoforms based on Gradiflow™ preparative electrophoresis technology is described. Gradiflow™ electrophoresis is scaleable, and incorporation into glycoprotein biopharmaceutical production bioprocesses as a potential final step facilitates the production of biopharmaceutical preparations of improved in vivo potency.

Keywords
ART, assisted reproductive technique; EACA, 6-aminohexanoic acid; ET, subsequent embryo transfer; FSH, follicle stimulating hormone; GABA, 4-aminobutyric acid; HEPES, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; IVF, in vitro fertilisation; MES, 2-
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Fractionation of follicle stimulating hormone charge isoforms in their native form by preparative electrophoresis technology
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 122, Issue 1, 9 March 2006, Pages 73–85
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us