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Use of glucose consumption rate (GCR) as a tool to monitor and control animal cell production processes in packed-bed bioreactors

Paper ID Volume ID Publish Year Pages File Format Full-Text
25582 43582 2006 8 PDF Available
Title
Use of glucose consumption rate (GCR) as a tool to monitor and control animal cell production processes in packed-bed bioreactors
Abstract

For animal cell cultures growing in packed-bed bioreactors where cell number cannot be determined directly, there is a clear need to use indirect methods that are not based on cell counts in order to monitor and control the process.One option is to use the glucose consumption rate (GCR) of the culture as an indirect measure to monitor the process in bioreactors.This study was done on a packed-bed bioreactor process using recombinant CHO cells cultured on Fibra-Cel® disk carriers in perfusion mode at high cell densities. A key step in the process is the switch of the process from the cell growth phase to the production phase triggered by a reduction of the temperature. In this system, we have used a GCR value of 300 g of glucose per kilogram of disks per day as a criterion for the switch.This paper will present results obtained in routine operations for the monitoring and control of an industrial process at pilot-scale. The process operated with this GCR-based strategy yielded consistent, reproducible process performance across numerous bioreactor runs performed on multiple production sites.

Keywords
Bioreactor; CHO; Fibra-Cel®; Glucose consumption rate; Packed-bed; Perfusion
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Use of glucose consumption rate (GCR) as a tool to monitor and control animal cell production processes in packed-bed bioreactors
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 122, Issue 1, 9 March 2006, Pages 122–129
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us