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Considerations on the lactate consumption by CHO cells in the presence of galactose

Paper ID Volume ID Publish Year Pages File Format Full-Text
25595 43583 2006 10 PDF Available
Title
Considerations on the lactate consumption by CHO cells in the presence of galactose
Abstract

A CHO cell line producing t-PA was cultured using glutamate and glucose or galactose to decrease the formation of metabolic end-products and therefore improving the process. In batch cultures using glutamate (6 mM) with glucose at two different levels (5 and 20 mM) or with glucose and galactose (5 and 20 mM, respectively) a remarkable difference in cell culture parameters was evidenced. For 20 mM glucose, a usual cell pattern was observed with lactate built-up in the medium. For 5 mM glucose, cell growth was arrested due to glucose depletion and only a limited use of the excreted lactate could be observed, not supporting cell growth sufficiently. However, when glucose 5 mM and galactose 20 mM were used together, cells consumed the glucose first and, interestingly, in a second phase they continued growing on galactose with the simultaneous consumption of the endogenous lactate. Under these conditions, cell growth was even improved with respect to growth on 20 mM glucose, used as a control. This metabolic behavior is further investigated by using metabolic flux analysis, suggesting that the lactate produced is not used in the oxidative metabolism through the TCA cycle. Metabolic fate of the lactate consumed is discussed.

Keywords
CHO cells; Glucose; Lactate; Galactose; Glutamate; t-PA
First Page Preview
Considerations on the lactate consumption by CHO cells in the presence of galactose
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 125, Issue 4, 1 October 2006, Pages 547–556
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering