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Direct capture of plasmid DNA from non-clarified bacterial lysate using polycation-grafted monoliths

Paper ID Volume ID Publish Year Pages File Format Full-Text
25609 43584 2006 13 PDF Available
Title
Direct capture of plasmid DNA from non-clarified bacterial lysate using polycation-grafted monoliths
Abstract

Monolith columns from macroporous polyacrylamide gel were grafted with polycations, poly(N,N-dimethylaminoethyl methacrylate) (polyDMAEMA), (2-(methacryloyloxy)ethyl)-trimethyl ammonium chloride (polyMETA) and partially quaternized polyDMAEMA prepared via treating polyDMAEMA-grafted columns with propylbromide. The polymer grafting degrees varied between 34 and 110%. The polycation-grafted monolithic columns are able to capture plasmid DNA directly from alkaline lysate of Escherichia coli cells. Due to the large pore size in macroporous monoliths the particulate material present in non-clarified feeds did not block the columns. The captured plasmid DNA was eluted with 1 M NaCl as particulate-free preparation with significantly reduced content of protein and RNA as compared to the applied lysate.

Keywords
Supermacroporous gel; Monolith column; Plasmid DNA; Tentacle chromatography
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Direct capture of plasmid DNA from non-clarified bacterial lysate using polycation-grafted monoliths
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 123, Issue 3, 29 May 2006, Pages 343–355
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
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Price after discount Only $4.95
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