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On-line low-volume transesterification-based assay for immobilized lipases

Paper ID Volume ID Publish Year Pages File Format Full-Text
25621 43585 2006 11 PDF Available
Title
On-line low-volume transesterification-based assay for immobilized lipases
Abstract

A method has been developed for fast evaluation of transesterification activity of immobilized lipases using microlitre and submicrolitre volumes of substrate solutions. The model reaction (acylation of isopropanol with vinyl acetate) is catalyzed by microbial lipases immobilized on ceramic particles, diatoms or acrylic resin, packed into a Teflon tube connected to fused-silica capillary tubing. The substrate solution is pumped through the microreactor and the product of transesterification, acetaldehyde, quantified on capillary by UV absorbance at 280 nm. Using this system in the continuous-flow mode, comparisons were made of transesterification catalysed by PS-C II lipase in two different solvent mixtures. Acetonitrile was found to be a compatible solvent that can be used as a solubilizer without suppressing enzymatic activity. The method has been used to compare conversions at a fixed flow rate using a single pass of substrate through packed beds containing ∼0.2 mg of supported enzyme. Spatial distributions of the product have been visualized using a complementary metal oxide semiconductor (CMOS) imaging detector in conjunction with the microreactor system operated in the stopped-flow mode.

Keywords
AcH, acetaldehyde; CA-AR, Candida antarctica B lipase (CAL-B) immobilized on acrylic resin; CMOS, complementary metal oxide semiconductor; CCD, charge coupled device; EC, enzyme commission; FWHM, full width at half maximum; PS-C, Pseudomonas cepacia lipas
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On-line low-volume transesterification-based assay for immobilized lipases
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 126, Issue 4, 1 December 2006, Pages 508–518
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us