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Disruption of hexokinase II (HXK2) partly relieves glucose repression to enhance production of human kringle fragment in gratuitous recombinant Saccharomyces cerevisiae

Paper ID Volume ID Publish Year Pages File Format Full-Text
25626 43585 2006 6 PDF Available
Title
Disruption of hexokinase II (HXK2) partly relieves glucose repression to enhance production of human kringle fragment in gratuitous recombinant Saccharomyces cerevisiae
Abstract

The GAL1 gene encoding galactokinase was disrupted in a recombinant Saccharomyces cerevisiae strain in which production of LK8 protein, a kringle fragment of human apolipoprotein, is under the control of GAL1 promoter. Null mutation of the HXK2 gene was introduced further in the gal1Δ strain to relieve glucose repression. A pattern for LK8 expression was compared for the two recombinant S. cerevisiae systems in continuous and fed-batch cultivations. A critical dilution rate in continuous cultivation that repressed LK8 expression was significantly higher for the gal1Δhxk2Δ strain than that for the gal1Δ strain to sustain the LK8 production even at high glucose consumption rate. Expressed LK8 for the gal1Δ strain was not detectable when the dilution rate exceeded 0.05 h−1. Maximum LK8 concentration of 57 mg l−1 was obtained in glucose-limited fed-batch cultivation of the gal1Δhxk2Δ strain, corresponding to a 13.8-fold enhancement compared with the gal1Δ strain grown under the same conditions.

Keywords
GAL1; Glucose repression; HXK2; Kringle; Saccharomyces cerevisiae
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Disruption of hexokinase II (HXK2) partly relieves glucose repression to enhance production of human kringle fragment in gratuitous recombinant Saccharomyces cerevisiae
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Biotechnology - Volume 126, Issue 4, 1 December 2006, Pages 562–567
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us